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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
05/12/2016 |
Actualizado : |
07/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CARLSON, J. S.; GIANNITTI, F.; VALKIÜNAS, G.; TELL, L. A.; SNIPES, J.; WRIGHT, S.; CORNEL, A. J. |
Afiliación : |
JENNY S. CARLSON, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of California, Davis, CA, USA; GEDIMINAS VALKIÜNAS, Nature Research Centre, Akademijos 2; LISA A. TELL, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; JOY SNIPES, Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California; STAN WRIGHT, Sacramento-Yolo Mosquito Vector and Control District; ANTHONY J. CORNEL, Mosquito Control Research Laboratory, Department of Entomology and Nematology, Kearney Agriculture Center, University of California; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California. |
Título : |
A method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays. |
Complemento del título : |
Methodology. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Malaria Journal, 2016, v.15 (5): Article number 1198. OPEN ACCESS |
ISSN : |
1475-2875 |
DOI : |
10.1186/s12936-016-1198-5 |
Idioma : |
Inglés |
Notas : |
Article history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. |
Contenido : |
ABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds.
© 2016 Carlson et al. MenosABSTRACT.
Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated int... Presentar Todo |
Palabras claves : |
ANIMAL EXPERIMENT; AVIAN MALARIA; BIRD INOCULATION; BLOOD PRESERVATION; CULEX spp. VECTORS; EXPERIMENTAL INFECTION; PATHOLOGY; PLASMODIUM CATHEMERIUM. |
Thesagro : |
MODELOS ANIMALES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12151/1/s12936-016-1198-5.pdf
https://malariajournal.biomedcentral.com/articles/10.1186/s12936-016-1198-5
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Marc : |
LEADER 03718naa a2200337 a 4500 001 1056201 005 2018-12-07 008 2016 bl uuuu u00u1 u #d 022 $a1475-2875 024 7 $a10.1186/s12936-016-1198-5$2DOI 100 1 $aCARLSON, J. S. 245 $aA method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 08 July 2015 // Accepted 01 March 2016 //First Online 11 March 2016. 520 $aABSTRACT. Background: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes. Currently, there is only one described method for long-term storage of Plasmodium spp. - infected wild avian blood and it is reliable at a parasitaemia of at least 1 %. However, most naturally infected wild-caught birds have a parasitaemia of much less that 1 %. To address this problem, a method for short-term storage of infected wild avian blood with low parasitaemia (even ?0.0005 %) has been explored and validated. Methods: To obtain viable infective merozoites, blood was collected from wild birds using a syringe containing the anticoagulant and the red blood cell preservative citrate phosphate dextrose adenine solution (CPDA). Each blood sample was stored at 4°C for up to 48 h providing sufficient time to determine the species and parasitaemia of Plasmodium spp. in the blood by morphological examination before injecting into donor canaries. Plasmodium spp. - infected blood was inoculated intravenously into canaries and once infection was established, Culex stigmatosoma, Cx. pipiens and Cx. quinquefasciatus mosquitoes were then allowed to feed on the infected canaries to validate the efficacy of this method for mosquito vector competence assays. Results: Storage of Plasmodium spp. - infected donor blood at 4°C yielded viable parasites for 48 h. All five experimentally-infected canaries developed clinical signs and were infectious. Pathologic examination of three canaries that later died revealed splenic lesions typical of avian malaria infection. Mosquito infectivity assays demonstrated that Cx. stigmatosoma and Cx. pipiens were competent vectors for Plasmodium cathemerium. Conclusions: A simple method of collecting and preserving avian whole blood with malaria parasites of low parasitaemia (?0.0005 %) was developed that remained viable for further experimental bird and mosquito infectivity assays. This method allows researchers interested in conducting infectivity assays on target Plasmodium spp. to collect these parasites directly from nature with minimal impact on wild birds. © 2016 Carlson et al. 650 $aMODELOS ANIMALES 653 $aANIMAL EXPERIMENT 653 $aAVIAN MALARIA 653 $aBIRD INOCULATION 653 $aBLOOD PRESERVATION 653 $aCULEX spp. VECTORS 653 $aEXPERIMENTAL INFECTION 653 $aPATHOLOGY 653 $aPLASMODIUM CATHEMERIUM 700 1 $aGIANNITTI, F. 700 1 $aVALKIÜNAS, G. 700 1 $aTELL, L. A. 700 1 $aSNIPES, J. 700 1 $aWRIGHT, S. 700 1 $aCORNEL, A. J. 773 $tMalaria Journal, 2016$gv.15 (5): Article number 1198. OPEN ACCESS
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
11/11/2014 |
Actualizado : |
11/05/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
MONTOSSI, F.; HODGSON, J.; MORRIS, S.T.; RISSO, D.; GORDON, I.L. |
Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JONATHAN M. HODGSON, College of Sciences, Massey University, Palmerston North, New Zealand; S. T. MORRIS, College of Sciences, Massey University, Palmerston North, New Zealand; DIEGO FERNANDO RISSO RIET, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; I. L. GORDON, College of Sciences, Massey University, Palmerston North, New Zealand. |
Título : |
A comparative study of herbage intake, ingestive behaviour and diet selection, and effects of condensed tannins upon body and wool growth in lambs grazing Yorkshire fog (Holcus lanatus) and annual ryegrass (Lolium multiflorum) dominant swards. |
Fecha de publicación : |
2001 |
Fuente / Imprenta : |
Journal of Agricultural Science, 2001, v. 136, no. 2, p. 241-251. DOI: https://doi.org/10.1017/S0021859601008668 |
DOI : |
10.1017/S0021859601008668 |
Idioma : |
Inglés |
Notas : |
Article history: Revised MS received 19 April 2000 // .Published online by Cambridge University Press 25 April 2001. |
Contenido : |
An experiment was carried out from August to early November 1994 to examine differences in diet selection, herbage intake, grazing behaviour and animal performance between weaned lambs rotationally grazing swards of annual ryegrass (Lolium multiflorum)}white clover (Trifolium repens)
and Yorkshire fog (Holcus lanatus)}T. repens with or without Lotus corniculatus. There were four replicate groups of six lambs per treatment. The effects of condensed tannins (CT) on lamb production were assessed by twice-daily oral administration of 10 g polyethylene glycol (PEG; molecular weight 4000) to half the lambs on each sward. The Lotus content of all swards was very low, and results are presented here for main sward comparisons meaned over lotus treatments. Overall mean estimates of pre-grazing herbage mass and sward surface height for the annual ryegrass and Yorkshire fog swards respectively, were 5820 v. 4360³190 kg DM}ha (P!0±001) and 29 v. 21³0±6 cm (P!0±001). The coefficient of organic matter digestibility (OMD) of the diet selected and herbage intake were higher on Yorkshire fog than on annual ryegrass (0±78 v. 0±74³0±080 g}kg; P!0±05, and 1070 v. 860³57 g OM per lamb per day, P!0±05 respectively), reflecting the higher content in the diet of grass green leaf (980 v. 930 g}kg³14 g}kg, P!0±05) and the lower content of dead material (80 v. 110³15 g}kg, P!0±08). Lambs grazing on Yorkshire fog swards had higher clean wool growth rate (1470 v. 1280³30 mg}cm per day, P!0±01) and greater fibre diameter (31 v. 29³0±2 µ, P!0±001), greater liveweight gain (152 v. 108³5±5 g}day, P!0±001), final weight (42 v.
38³0±5 kg, P!0±001), carcass weight gain (89 v. 69³2±5 g}day, P!0±001), carcass weight (19 v. 17³0±3 kg, P!0±001) and soft tissue thickness (GR value 11 v. 8³0±5 mm, P!0±01), and lower faecal egg counts (FEC; square root transferred values 9±2 v. 11±0³0±4 eggs}g fresh faeces, P!0±01)
than lambs grazing annual ryegrass swards. Similar dietary concentrations of condensed tannins (CT) between Yorkshire fog and annual ryegrass swards (4±2 v. 3±7 DM³0±2 g}kg, P!0±08) increased clean wool growth (1440 v. 1310³32 mg}cm# per day, P!0±05), fibre diameter (30±7 v. 29±5³0±21 µ,
P!0±01) and liveweight gain (141 v. 120³4±3 g per lamb per day, P!0±01), although differences in carcass weight (17±9 v. 18±2³0±3 kg) and FEC transformed values (9±6 v. 11±0³0±6 eggs}g fresh faeces) were not significant. The effects of CT on animal performance were greater in Yorkshire fog
swards. CT had no significant effects on diet selection, herbage intake and grazing behaviour patterns. MenosAn experiment was carried out from August to early November 1994 to examine differences in diet selection, herbage intake, grazing behaviour and animal performance between weaned lambs rotationally grazing swards of annual ryegrass (Lolium multiflorum)}white clover (Trifolium repens)
and Yorkshire fog (Holcus lanatus)}T. repens with or without Lotus corniculatus. There were four replicate groups of six lambs per treatment. The effects of condensed tannins (CT) on lamb production were assessed by twice-daily oral administration of 10 g polyethylene glycol (PEG; molecular weight 4000) to half the lambs on each sward. The Lotus content of all swards was very low, and results are presented here for main sward comparisons meaned over lotus treatments. Overall mean estimates of pre-grazing herbage mass and sward surface height for the annual ryegrass and Yorkshire fog swards respectively, were 5820 v. 4360³190 kg DM}ha (P!0±001) and 29 v. 21³0±6 cm (P!0±001). The coefficient of organic matter digestibility (OMD) of the diet selected and herbage intake were higher on Yorkshire fog than on annual ryegrass (0±78 v. 0±74³0±080 g}kg; P!0±05, and 1070 v. 860³57 g OM per lamb per day, P!0±05 respectively), reflecting the higher content in the diet of grass green leaf (980 v. 930 g}kg³14 g}kg, P!0±05) and the lower content of dead material (80 v. 110³15 g}kg, P!0±08). Lambs grazing on Yorkshire fog swards had higher clean wool growth rate (1470 v. 1280³30 mg}cm per day, P!0±01) and greate... Presentar Todo |
Thesagro : |
GANADO BOVINO; PASTURAS; TANINOS. |
Asunto categoría : |
L01 Ganadería |
Marc : |
LEADER 03605naa a2200229 a 4500 001 1051564 005 2020-05-11 008 2001 bl uuuu u00u1 u #d 024 7 $a10.1017/S0021859601008668$2DOI 100 1 $aMONTOSSI, F. 245 $aA comparative study of herbage intake, ingestive behaviour and diet selection, and effects of condensed tannins upon body and wool growth in lambs grazing Yorkshire fog (Holcus lanatus) and annual ryegrass (Lolium multiflorum) dominant swards. 260 $c2001 500 $aArticle history: Revised MS received 19 April 2000 // .Published online by Cambridge University Press 25 April 2001. 520 $aAn experiment was carried out from August to early November 1994 to examine differences in diet selection, herbage intake, grazing behaviour and animal performance between weaned lambs rotationally grazing swards of annual ryegrass (Lolium multiflorum)}white clover (Trifolium repens) and Yorkshire fog (Holcus lanatus)}T. repens with or without Lotus corniculatus. There were four replicate groups of six lambs per treatment. The effects of condensed tannins (CT) on lamb production were assessed by twice-daily oral administration of 10 g polyethylene glycol (PEG; molecular weight 4000) to half the lambs on each sward. The Lotus content of all swards was very low, and results are presented here for main sward comparisons meaned over lotus treatments. Overall mean estimates of pre-grazing herbage mass and sward surface height for the annual ryegrass and Yorkshire fog swards respectively, were 5820 v. 4360³190 kg DM}ha (P!0±001) and 29 v. 21³0±6 cm (P!0±001). The coefficient of organic matter digestibility (OMD) of the diet selected and herbage intake were higher on Yorkshire fog than on annual ryegrass (0±78 v. 0±74³0±080 g}kg; P!0±05, and 1070 v. 860³57 g OM per lamb per day, P!0±05 respectively), reflecting the higher content in the diet of grass green leaf (980 v. 930 g}kg³14 g}kg, P!0±05) and the lower content of dead material (80 v. 110³15 g}kg, P!0±08). Lambs grazing on Yorkshire fog swards had higher clean wool growth rate (1470 v. 1280³30 mg}cm per day, P!0±01) and greater fibre diameter (31 v. 29³0±2 µ, P!0±001), greater liveweight gain (152 v. 108³5±5 g}day, P!0±001), final weight (42 v. 38³0±5 kg, P!0±001), carcass weight gain (89 v. 69³2±5 g}day, P!0±001), carcass weight (19 v. 17³0±3 kg, P!0±001) and soft tissue thickness (GR value 11 v. 8³0±5 mm, P!0±01), and lower faecal egg counts (FEC; square root transferred values 9±2 v. 11±0³0±4 eggs}g fresh faeces, P!0±01) than lambs grazing annual ryegrass swards. Similar dietary concentrations of condensed tannins (CT) between Yorkshire fog and annual ryegrass swards (4±2 v. 3±7 DM³0±2 g}kg, P!0±08) increased clean wool growth (1440 v. 1310³32 mg}cm# per day, P!0±05), fibre diameter (30±7 v. 29±5³0±21 µ, P!0±01) and liveweight gain (141 v. 120³4±3 g per lamb per day, P!0±01), although differences in carcass weight (17±9 v. 18±2³0±3 kg) and FEC transformed values (9±6 v. 11±0³0±6 eggs}g fresh faeces) were not significant. The effects of CT on animal performance were greater in Yorkshire fog swards. CT had no significant effects on diet selection, herbage intake and grazing behaviour patterns. 650 $aGANADO BOVINO 650 $aPASTURAS 650 $aTANINOS 700 1 $aHODGSON, J. 700 1 $aMORRIS, S.T. 700 1 $aRISSO, D. 700 1 $aGORDON, I.L. 773 $tJournal of Agricultural Science, 2001$gv. 136, no. 2, p. 241-251. DOI: https://doi.org/10.1017/S0021859601008668
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